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Laboratory of Ecochemistry and Ecotoxicology

Scientific Supervisor: prof. dr hab. Maria Niklińska

Supervisor of the Chromatography Lab (labs 3.0.1-3.0.4): Maciej Choczyński, MSc. Eng.

Supervisor of the AAS Lab (2.1.12): Paweł Dudzik, MSc. 

Supervisor of the Ecotoxicology Lab (labs 2.1.9 - 2.1.11): Ligia Kuriańska-Piątek, MSc.

 

Detailed information about the equipment, the analyses performed, and their costs can be found on the Laboratory SharePoint (accessible by logging into the UJ domain):

https://ujchmura.sharepoint.com/teams/ekochemLABUJ

Please direct any questions, orders, etc. to Ms. Małgorzata Kinasiewicz, M.Sc.:

malgorzata.kinasiewicz@uj.edu.pl

phone: +48 12 664 5985

  • GC-MS with a complete solvent-free extraction system for volatile substances from liquid and gaseous materials (Perkin Elmer, Clarus600 equipped with mass detector Clarus 600C + headspace injector Turbo Matrix XS attachment)
  • HPLC-MS/MS Agilent 1260 Infinity II with mass detector Agilent 6470 B
  • CHNOS elemental analyser (Elementar Vario El III)
  • AAS with graphite cuvette and flame (Perkin-Elmer AAnalyst 800, Perkin-Elmer AAnalyst 200, Perkin-Elmer PinAAcle 900Z)
  • Perkin-Elmer Titan MPS microwave sample digestion system
  • μQuant (BioTek) and Synergy HTX (BioTek) microplate readers
  • Micro-Oxymax microrespirometer (Columbus Instruments)
  • Flow injection analyser with visible light detector (FIA compact, MLE GmbH)
  • Kjeldahl analyser with mineralizer for nitrogen determination
  • Image recording and analysis kit (Olympus binocular microscope with Canon EOS 90D digital camera)
  • Spray tower (Potter Precision Laboratory Spray Tower, Burkard)
  • Ovens, dryers, mineralizers, homogenizers, hot plates, shakers, pH meters, analytical balances, Tullgren funnels, etc.
  • Precise laboratory multifunction meter for physic-chemical measurements (Elmetron, CX-601) equipped with chloride and conductometric electrodes
  • Sample preparation: ball homogenizer for 2 ml, 7 ml and 30 ml tubes (Bead Ruptor), dynamic homogenizer stomacher type (Interscience, MiniMix 100 CC), tissue homogenizer (Omni TH-02), heating thermomixer (Hettich Benelux, MHR 13) automatic extractor for accelerated extraction of solid samples under high pressure and high temperature conditions (Thermo Scientific Dionex ASE 350), automatic 6-channel SPE extraction system (Lab Tech), system for rapid evaporation of solvents in a stream of nitrogen (Labconco RapidVap Vertex), centrifuge up to 4000 rcf, 6 X 15 ml and 6 X 50 ml (Ohaus, Frontier 5706), ultrasonic bath, ultrasonic power 860 W (Bandelin Sonorex, Digitec DT 512H)
  • Climate chambers (temperature, humidity and lighting control)

The Laboratory of Ecochemistry and Ecotoxicology provides services mainly to units of the Faculty of Biology of the Jagiellonian University. When possible, we also offer services to external units. Examples of analyses performed in our laboratories:

Gas and liquid chromatography:

  • determination of pesticides in biological materials
  • determination of phospholipid fatty acids (PLFA) in soil
  • determination of hormones in biological materials

Elemental analyzer:

  • determination of carbon, nitrogen and sulfur in biological samples and soil

Atomic absorption and emission spectrometry:

  • determination of metal content in biological samples and soil

Microplate colorimeter/densitometer:

  • analysis of functional diversity of soil microorganisms
  • determination of protein content in biological materials
  • determination of enzymatic reaction rates

Flow injection analyzer:

  • determination of phosphorous in ortophosphate form and nitrogen in nitrate and nitrite forms in extracts and mineralizates